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文章摘要
YAN Chang-yu.Simultaneous Determination of Six Alkaloids in Guiling Tablets by HPLC[J].中国药物评价,2019,36(3):184-188
HPLC法同时测定桂灵片中6种生物碱的含量
Simultaneous Determination of Six Alkaloids in Guiling Tablets by HPLC
投稿时间:2019-04-16  修订日期:2019-06-18
DOI:
中文关键词: 桂灵片  吗啡  盐酸麻黄碱  盐酸伪麻黄碱  盐酸甲基麻黄碱  磷酸可待因  盐酸罂粟碱
英文关键词: Guiling Tablets  Morphine  Ephedrine  Pseudoephedrine  Methylephedrine hydrochloride  Codeine  Papaverine
基金项目:2017年国家药典委员会药品标准提高课题(项目编号:428)
作者单位E-mail
鄢长余 大连市检验检测认证技术服务中心 125743509@qq.com 
赵月然 大连市检验检测认证技术服务中心 moonzyr@yeah.net 
姜范成 大连市检验检测认证技术服务中心  
季雪 大连市检验检测认证技术服务中心  
李宪刚 大连市检验检测认证技术服务中心  
周凌 大连市检验检测认证技术服务中心  
高珊 大连市检验检测认证技术服务中心  
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中文摘要:
      目的:建立HPLC法同时测定桂灵片中6种生物碱的含量。方法:采用Agilent Eclipse Plus-C18色谱柱(4.6 mm×250 mm,4.6 μm),以乙腈(A)-0.1%磷酸(B)为流动相,梯度洗脱,流速1.0 mL·min-1,测定波长:吗啡、盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、磷酸可待因210 nm;盐酸罂粟碱251 nm。结果:吗啡、盐酸麻黄碱、盐酸伪麻黄碱、盐酸甲基麻黄碱、磷酸可待因和盐酸罂粟碱进样量分别在0.001 3~0.13 μg(r=0.999 9),0.002 5~0.25 μg(r=0.999 9),0.002 5~0.25 μg(r=0.999 9),0.005 4~0.27 μg(r=0.999 9),0.002 6~0.26 μg(r=0.999 9),0.000 5~0.11 μg(r=0.999 9)范围内呈良好线性关系,平均回收率(n=6)分别为101.6%、100.2%、95.3%、99.2%、103.5%、100.2%。结论:该方法可以用于桂灵片的质量控制。
英文摘要:
      Objective:To establish an HPLC method for simultaneous determination of six alkaloids in Guiling tablets,and to analyze the results by principal component analysis. Methods: Separation was performed on a Agilent Eclipse Plus-C18 column(4.6 mm×250 mm,5 μm).And the mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution at a flow rate of 1.0mL·min-1.The detection wavelength was 210 nm for morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and 251 nm for papaverine. Results:Morphine、ephedrine、pseudoephedrine、methylephedrine hydrochloride、codeine and papaverine showed good linearity in the ranges of 0.001 3~0.13 μg(r=0.999 9), 0.002 5~0.25 μg(r=0.999 9), 0.002 5~0.25 μg(r=0.999 9), 0.005 4~0.27 μg(r=0.999 9), 0.002 6~0.26 μg(r=0.999 9), 0.000 5~0.11 μg(r=0.999 9).The average recoveries(n=6) were 101.6%、 100.2%、95.3%、99.2%、103.5%、100.2%. Principal component analysis extracted two principal components,and explained 91.7% of the group differences, which better reflected the difference between groups. Conclusion:The method is applicable for the quality control of Guiling tablets.
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