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BIN LIU.Simultaneous Determination of Six Kinds of Components in Longdanshugan Capsules by HPLC-DAD[J].中国药物评价,2019,36(4):258-263
HPLC-DAD法同时测定龙胆舒肝胶囊中6种活性成分的含量
Simultaneous Determination of Six Kinds of Components in Longdanshugan Capsules by HPLC-DAD
投稿时间:2019-04-22  修订日期:2019-07-19
DOI:
中文关键词: 龙胆舒肝胶囊  天麻素  龙胆苦苷  芍药苷  毛蕊花糖苷  迷迭香酸  丹酚酸B  高效液相色谱-二极管阵列检测器
英文关键词: Longdanshugan capsules  Gastrodin  Gentiopicroside  Paeoniflorin  Acteoside  Rosmarinic acid  Salvianolic acid B  HPLC-DAD
基金项目:
作者单位E-mail
刘斌 泰安市食品药品检验检测中心 liubin8247@sina.com 
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中文摘要:
      目的:建立HPLC-DAD法同时测定龙胆舒肝胶囊中天麻素、龙胆苦苷、芍药苷、毛蕊花糖苷、迷迭香酸和丹酚酸B含量的方法。方法:采用液相色谱法,色谱柱为Waters Symmetry ShieldTM RP18,流动相为乙腈(A)和0.1%的磷酸水溶液(B),梯度洗脱;流速:1.0 mL·min-1;柱温:35 ℃;检测波长:天麻素、毛蕊花糖苷、迷迭香酸和丹酚酸B为220 nm,芍药苷230 nm,龙胆苦苷为275 nm。结果:天麻素、龙胆苦苷、芍药苷、毛蕊花糖苷、迷迭香酸和丹酚酸B的线性范围分别为3.13~62.6 μg·mL-1(r=0.999 5),4.12~82.4 μg·mL-1(r=0.999 9),6.60~132.0 μg·mL-1(r=0.999 7),1.66~33.2 μg·mL-1(r=0.999 5),3.54~70.8 μg·mL-1(r=0.999 8)和4.65~93.0 μg·mL-1(r=0.999 6),6种成分的平均加样回收率(n=6)分别为97.8%、98.0%、98.6%、98.1%、98.7%和98.1%,RSD分别为1.9%、1.3%、1.6%、1.0%、1.3%和1.0%。结论:本法操作简便,结果准确,重现性好,可用于龙胆舒肝胶囊的质量控制。
英文摘要:
      Objective:To establish a HPLC-DAD method for determination of gastrodin, gentiopicroside, paeoniflorin, acteoside, rosmarinic acid and salvianolic acid B in Longdanshugan capsules. Methods:A stable HPLC method was established and the chromatography was accomplished on a Waters Symmetry ShieldTM RP18 column, the mobile phase consisted of acetonitrile (A)-0.1% (w)phosphoric acid(B) as mobile phase in a gradient mode. The flow rate was 1.0 mL·min-1 and the column temperature was set at 35 ℃. The detection wavelength was set at 220 nm for gastrodin, acteoside,rosmarinic acid and salvianolic acid B, 230 nm for paeoniflorin, 275 nm for gentiopicroside,respectively. Results:The linear ranges of gastrodin, gentiopicroside, paeoniflorin, acteoside, rosmarinic acid and salvianolic acid B were 3.13-62.6 μg·mL-1(r=0.999 5), 4.12-82.4 μg·mL-1(r=0.999 9), 6.60-132.0 μg·mL-1(r=0.999 7), 1.66-33.2μg·mL-1(r=0.9995), 3.54-70.8 μg·mL-1 (r=0.999 8) and 4.65-93.0 μg·mL-1(r=0.999 6), respectively. The average recovery were 97.8%, 98.0%, 98.6%, 98.1%, 98.7% and 98.1%, RSD were 1.9%, 1.3%, 1.6%, 1.0%, 1.3% and 1.0%. Conclusion:The method is simple to operate,accurate and reproducible, which can be used for the quality control of Longdanshugan capsules.
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